Evaluated by Western Blotting in HEK293 transfected with pFin-EF1-mCherry vector. This antibody has been used to image mCherry fusion proteins expressed in HEK293T cells via western blot with high rates of success.Antibody was diluted in 3% milk TBST and incubated overnight at 4 degrees. 8 hpi, infection was quantified by measuring Fluc reporter . Contact Us. . Handling. Validated Applications: WB, ICC/IF and IHC. since the western blot result of both protein . Independently reviewed in 3 review(s). MCherry antibody LS-C784928 is an unconjugated rabbit polyclonal antibody to mCherry. If anyone is still looking for a reliable anti-mCherry antibody, you might want to try the guinea pig anti-rfp from Synaptic System (#390 004). This antibody recognizes very well tdTomato and does not recognize GFP (green fluorescent protein). Product Details. Supplied as 100 L purified antibody (2 mg/mL). Western blot - Anti-mCherry antibody (ab167453) Buy rabbit polyclonal antibody to mCherry (A85306). mCherry Antibody (clone 1C51) LS . A western blot analysis of the recombinant His-Erc1-Myc-His protein with antibodies specific for the His-tag and Myc-tag showed that, while the upper protein band was detectable both with an . Because this antibody is raised in mouse this means that an anti-mouse secondary antibody must be used for it's detection. . Rabbit Polyclonal mCherry antibody. . Better visualize your results with affinity-purified, monoclonal and polyclonal antibodies raised against whole RFP and GFP. . . mCherry Antibody LSC204826. Validated for WB. Target description ~32 kDa observed; 25.93 kDa calculated. 30 g whole cell lysate/extract of mCherry-tagged protein expressing 293T cells 5% SDS-PAGE The antibody solution should be gently mixed before use. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. MCherry antibody LS-C746473 is an unconjugated mouse monoclonal antibody to mCherry. [GT857] detects mCherry protein by western blot analysis. Detect mCherry protein using this chicken polyclonal anti-mCherry Antibody, Cat. Western blot analysis using recombinant mCherry. Western blot analysis was performed using KCC2 Antibody. In order to effectively pull down the mCherry fusion protein DNA complex, ChromoTek's RFP-Trap was used. Detect mCherry protein using this chicken polyclonal anti-mCherry Antibody, Cat. FIGURE 1 . Western blotting (0.5-4 g/ml). Catalog # Availability Size / Price Qty; AF2085: AF2085-SP: Add to Cart. mCherry in its monomeric form is useful for applications such as Frster Resonance Energy . (Courtesy of Dr Gerry Shaw). The REP transports the new Rab to a Rab geranyl-geranyl Uncharacterized bands may be observed in . However, in the first lane one can see best the unspecific bands at around 120 kDa . RRID AB . anti-Cherry fluorescent protein antibody, anti-red fluorescent protein mCherry antibody: Form/Appearance: PBS, 10% glycerol, 0.3% Sodium Azide . mCherry Antibody (clone 1C51) LS . The mCherry Antibody has been validated for the following applications: Western Blot. Price. Lane 1: 10 ng Lane 2: 20 ng Lane 3: 50 ng Lane 4: 100 ng . Cells were then infected with rMHV-A59 or rMHV-JHM viruses containing an Fluc reporter gene. . Product Images. View specifications, prices, citations, reviews, and more. ICC, Immunofluorescence, Western blot. CHO-K1 cells were stably transduced with the cDNA coding for mCherry-Fc fusion protein. In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. Biovision. . Western Blot Analysis: A representative lot detected mCherry in Western Blot applications (Krajcovic, M., et. Primary Antibody: Mouse Anti-mCherry Polyclonal Antibody (SPC-1268). Western blots were probed with specific anti-ULK1, anti-BECN1, anti-ATG5, anti-ATG12, anti-ATG16L1, anti-p62, and anti-PHB2 antibodies. 30 g 293T whole cell lysate/extract B. COVID-19. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. The Rab cycle. mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. Why has the mouse IgG light chain been detected in your Western blot data? Contact Us Call Us: +1 484.791.3823 Products. Request. . AF2085 118 Citations 2 Reviews Submit a Review Datasheet. . We recommend the following alternatives. The "-" lane is a blot of an equal amount of protein extract from untransfected HEK293 cells. Fig. Bio-Techne offers high-quality reagents instruments, custom manufacturing, and testing services. Background mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. Highly specific and rigorously validated in-house, mCherry (E5D8F) Rabbit Monoclonal Antibody (CST #43590) is ready to ship. ARG10759 anti-mCherry antibody [1C51] WB image. Lane 1: 1:5,000 Dilution. Validated for WB. VeriBlot for IP Detection Reagent . The Anti-mCherry Antibody [1C51] (A85305) does not react with GFP protein. - Find MSDS or SDS, a COA, data sheets and more information. According the attached image, The 2nd from the left . However, the optimal conditions should be determined individually. Western blot analysis of extracts from human, mouse, and rat brain membranes using KCC2 Antibody. Toggle navigation. mCherry was immunoprecipitated from 0.35 mg of 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged mCherry whole cell lysate with ab213511 at 1/20 dilution. Centrifuge briefly prior to opening the vial. The mCherry protein consists of 236 amino acid residues with a mass of 26.7 kilodaltons. Western Blot: Close. Supplemental Products. Bio Techne KO Validation; Atlas Antibodies Validation . The same blot was simultaneously probed with Anti-Heat Shock Protein 60 Antibody (A85438), at a dilution of 1:5,000, in red, which shows a band at 60 kDa seen only in cell lysates as a positive loading control. (C) Confocal microscopy of EGFP-mCherry-LC3B (yellow) expressed in treated NCM-460 cells, Sh-NLRP6, and Sh-ER cells. 1. Recombinant mCherry (Cat.#: 4993-100) can be used as a positive control. (A) DBT . PVDF membrane was probed with 0.25 g/mL of Rabbit Anti-Human mCherry Monoclonal Antibody (Catalog # MAB11041) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody . Western blot was performed from the immunoprecipitate using ab213511 at 1/1000 dilution. Western blotting (0.5-4 g/ml). The supernatant with the secreted protein of 57 kDa was collected and purified via Protein A affinity chromatography. (A) The EM25 PR reporter clone is an ECC1 clone derived from PR reporter plasmids only with M-cherry reporter. Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. No. Validated in WB, ICC/IF, IHC-Wm, IHC. Compare application images and datasheets for 26 anti mCherry-Tag- Antibody antibodies from 13 leading antibody suppliers, plus reviews and the top related antibodies. Because HEK293T cells have almost no base-line expression of KIF26A, the KIF26A expression detected in immunostaining and Western Blot is due to the expression of transfected constructs. The image is similar to the original image and for illustrative purposes only. His-tagged full-length recombinant MCherry. ARG55723 anti-mCherry antibody ICC/IF image Immunocytochemistry: 293HEK cells transfected with mCherry-Rab1a and stained with ARG55723 anti-mCherry antibody at 1:50 dilution. A . WB result of mCherry antibody (26765-1-AP; 1:2000; room temperature for 1.5 hours) with negatove control and mCherry overexpressed Transfected HEK-293 cells. 7 Images. HEK293 cells were transiently transfected with vectors encoding the following fluorescent proteins, and then analyzed by Western blot with the mCherry Monoclonal Antibody (0.5 mg/ml; 1:1000 dilution). MAB131873 detects the mCherry protein and is tested for use in immunocytochemistry and Western blotting. Catalog number. Anti-mCherry (Discosoma sp.) (D) Western blot of transfected HEK293T lysate showing expression of KIF26A and mCherry tag upon transfection of WT and patient variant KIF26A expression plasmids. Anti-mCherry, mCherry antibody, tag, AS18 4178. Western blotting of PR and its downstream genes after the treatment with the 5 top-picked drugs, Carfilzomib [100 nM], CUDC . Fusion proteins were detected using HA-tag . DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry Antibody. ARG10759 anti-mCherry antibody [1C51] binds a major band running at ~28 kDa corresponding to . Type Antibody Conjugate Unconjugated . Effect of tetraspanin antibodies on MHV and IAV infection. Mouse Monoclonal mCherry antibody [GT857]. Al. AB356481. A receptor that is crucial for the autophagy of mitochondria also mediates the autophagy of peroxisomes. Multiple PR reporter clones confirmed by PCR of P5 primer pairs and Western blotting by mCherry antibody. It is bright, photostable, and ideal for fluorescence microscopy. Reviews (4) Summary . Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, . Examples of mCherry antibody product reviews: In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. Citations (53) FAQs. Anti-mCherry Antibody, clone 1C51 Anti-mCherry Antibody, clone IC51, Cat. Product information: mCherry Antibody - Gentaur.com - Product info. . Immunoprecipitation of KCC2 from mouse brain extracts. mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. Recommended positive controls: 293T whole cell extract, human PLK4-mCherry-transfected 293T. Cited in 1 publication. It has also been shown to recognize dsRED . Compare Anti-mCherry Western Blot Antibody Products from leading suppliers on Biocompare. Anti-mCherry antibody, clone IC51, cat. Applications: ICC, Immunofluorescence, Western blot. mCherry Antibody LSC204826. In absence of suitable antibodies for an inducible hormone receptor, mCherry has been fused to the N-terminus of the receptor. Western blot: Crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector in lane labelled "+". It has been tested in Immunofluorescence and Western Blotting. However, the optimal conditions should be determined individually. mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. Applications: ICC, Immunofluorescence, Western blot. has been tested by ELISA and Western blot and is intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS). . Supplier. 5 reviews. Contact Us. Western blot analysis of mCherry recombinant protein, diluted at 1 . Enter the email address you signed up with and we'll email you a reset link. MCherry antibody LS-C204207 is an unconjugated goat polyclonal antibody to mCherry from discosoma. Product Details. (2013). Human/Mouse Brachyury Antibody . 26 matching mCherry Tag antibodies for Western Blot from 13 suppliers Close Superstar Kits. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. 2. AF3369 53 Citations 4 Reviews Submit a Review Datasheet. Citations (118) . Catalog # Availability Size / Price Qty; AF3369: AF3369-SP: Add to Cart. Mol Biol Cell . Direct-Blot HRP anti-mCherry Antibody - mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. Recognizes Red Fluorescent Protein (dsRed), tdTomato and mCherry and does not recognize GFP (green . mCherry Nanobody (1) mNeonGreen Western blot (1) mNeonGreen sequence (1) mNeonGreen-Trap (1) on-bead digestion . mCherry Antibodies. Resources. Monoclonal Antibody for studying mCherry bacteria. Researchers should determine optimal titers . J, K. Western blot analysis of soluble and insoluble fractions of RIPK2 +/+ and RIPK2 / HT-29 cells infected with S. flexneri (MOI 1:25 . Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry. A. (B) Coomassie gel and Western blot with anti-mCherry antibody. Validated in WB, ICC/IF, IHC. Cited in 22 reference(s). H, I. HEK293T cells were transfected with (H) full length and (I) CARD domains of NOD1, NOD2, and RIPK2 followed by Western blot analysis with soluble and insoluble fractions using indicated antibodies. Storage Conditions. mCherry is a second generation fluorescent protein and by far the most popular red emitting fluorescent protein due to its high photostability and size (the other photostable red fluorescent protein . The antibody works perfect. It has been tested in Immunofluorescence and Western Blotting. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence . United States (US) Country / Location Selection Product . Empty mCherry plasmid, mCherry-tagged WT human KIF26A and KIF26A patient variants expression plasmids were transfected with Lipofectatmine 3000 reagent. Cited in 3 reference(s). New Rab proteins are synthesized in the cytosol and recognized by a Rab escort protein (REP). Equal amounts of E. coli cell lysates with Thioredoxin(Trx) fusion proteins were used for analysis. Human/Mouse/Rat SOX1 Antibody . This mCherry antibody has been reported to recognise tdTomato although we have not independently tested this. Western Blot (WB) 1:1,000-1:10,000 10 Publications ChIP assay (ChIP) - 1 Publication . Species: Discosoma. Blue DNA stain. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mVISTA. Validation . . Mouse anti-mCherry antibody (clone 1C51) used for WB analysis of mCherry-ATG8 levels in larval muscles expressing 24B-Gal4-driven UAS-mCherry-ATG8 together with the control RNAi, the mask RNAi or UAS-Mask, at 93 h after egg laying. View 1 mCherry Primary Antibodies. Application. . MLKL, which displays plasma membrane (PM) pore-forming activity upon phosphorylation, fu. Application & Usage. Research Areas. mCherry is a monomeric red fluorescent protein (mRFP) with a theoretical molecular weight of 28 kDa belonging to the mFruits family. Necroptosis is a form of programmed necrotic cell death mediated by the kinase RIPK3 and its substrate MLKL. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is KCC2 Antibody. Lane 2: 1:10,000 Dilution. Detection of Human mCherry by Western Blot. Western blot: 100 g of 293HEK cells transduced with myc-mCherry Ad and stained with ARG55723 anti-mCherry antibody at 1:1000 dilution. The ratio of peak mCherry or EGFP signal to the average signal of a 1-m length of midbody flanking region immediately outside the boundary of the dark zone center (1.5 . We offer mCherry antibodies that have been cited . The blot was incubated with an antibody recognizing mCherry and RFP, the constructs are also detected (boxes). The following lists a number of MCherry antibodies from various sources. A representative Western blot using antibody raised against the RNAP subunit (RpoC) or the FLAG epitope, for wild-type untagged cells (first lane), nusB+ cells expressing SuhB-FLAG3 (second . Species: Discosoma. Invitrogen Anti-mCherry Monoclonal (16D7), Catalog # M11217. Western blot analysis of mCherry Recombinant Protein showing detection of ~28kDa mCherry protein using Mouse Anti-mCherry Polyclonal Antibody (SPC-1268). 5993-100. 25 l and 100 l of Direct-Blot HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. Rabbit anti mCherry antibody recognizes the red fluorescent mCherry protein, a monomeric engineered derivate of a fluorescent protein isolated from members of the Discosoma (mushroom coral) family. . Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. . No. -20 C. Recombinant mCherry (Cat.#: 4993-100) can be used as a positive control. Validated for IF, IHC and WB. Research Areas. . All antibodies used for Western blot analysis (WB), 3 immunocytochemistry (ICC), and immunoprecipitation (IP) are as follows: rabbit anti-ALIX (Santa Cruz Biotechnology sc . Resources. The antibody detects mCherry and mCherry tag fusion proteins. View application images and datasheets for 209 anti mCherry-Tag- Antibody antibodies from 16 leading antibody suppliers, plus reviews and the top related antibodies. (b) Western blot analysis of mCherry constructs expressed in E. coli cells. HEK-293 cells were subjected to SDS PAGE followed by western blot with 68088-1-Ig (mCherry antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Note that the lower molecular weight band of KIF26A is of the correct molecular size of KIF26A protein (194.6 kDa) and is therefore considered the authentic band used in quantification. AB356481. cells were treated with monoclonal antibodies to CD9, CD63, CD81, or an equimolar mixture of the three (Tspan) for 30 min. In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. Background levels of mCherry expression can be estimated from the fluorescence of the non-induced wild type construct (WT(NI)). The target antigen refers to the fluorescent protein tag, mCherry, which can be attached to recombinant proteins for detection. No. Image & description. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB). High Quality Low Prices 100% Guarantee Western blot. Validated for WB, IF, IF. Monosynaptic tracing analysis demonstrates the direct connectivity of Dmrt3 neurons to proprioceptors and brain centers involved in motor control, suggesting a sensorimotor integrative role for Dmrt3. Cited in 16 publications. The mCherry Monoclonal Antibody differentiates red fluorescent proteins The mCherry Monoclonal Antibody differentiates red fluorescent proteins. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting. The antibody was used at 1 : 1000 dilution and the data image shows free mCherry as well as ATG8 bound mCherry . The major band at about 30 kDa corresponds to mCherry protein. . Figure 1 . COVID-19. Antibody was used in 5% TBST overnight, gave a clean blot. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. 6 Images. Products. Store product as a concentrated solution. Toggle navigation. ICC, Immunofluorescence, Western blot. No.

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